My initial impression of Toy Story 3 (2010, directed by Lee Unkrich) was that it was more melancholy and not as funny as the previous entries in the series. I formed that impression about halfway through the movie. The second half of the movie dropped the bottom out of my assumptions. It still wasn't as "funny" as the other movies, but it turned into a film suffused by such existential terror that it is perhaps more frightening than anything in any recent horror movies. Oh, it's still a comedy. Sure. But the category, "Pixar comedy," has become an expansive one, loaded with every other kind of emotion to which human beings are prone, whether it's the intense romantic longing of WALL-E or the sadness and loss of Up or the sensory delight of Ratatouille.
Their palette has darkened considerably in Toy Story 3.